Evaluation of veterinary antibiotic residues in commercial and local farms in Al-Bayda city, Libya (2024-2025), with a molecular docking study to analyze their effect on target microbial proteins

Authors

  • Abdulsalam Saleh Department of Health Food Hygiene, Faculty of Veterinary Medicine, University of Omar Al-Mukhtar, EL-Beida, Libya
  • Rihab Ramadhan Farhat Department of Zoology, Faculty of Science, Omar al-Mukhtar University, EL-Beida, Libya
  • Zafir AM Zafir Department of Health Food Hygiene, Faculty of Veterinary Medicine, University of Omar Al-Mukhtar, EL-Beida, Libya
  • Randa Mahmoud Department of Health Food Hygiene, Faculty of Veterinary Medicine, University of Omar Al-Mukhtar, EL-Beida, Libya
  • Mohammd Abd Alati Department of Health Food Hygiene, Faculty of Veterinary Medicine, University of Omar Al-Mukhtar, EL-Beida, Libya
  • Hajir Othman Department of Biochemistry, Faculty of Medicine, University of Benghazi, Libya

Keywords:

antibiotic, eggs, milk, Neomycin, penicillin, enrofloxacin, Al-Bayda city.

Abstract

This study detected veterinary antibiotic residues (neomycin, penicillin, enrofloxacin) in 400 Libyan samples (200 eggs, 200 milk) using microbial inhibition and chromatographic techniques. Results showed significantly higher residues (p≤0.05) in local vs. commercial farms, with minimal reduction after boiling. Penicillin showed highest residues (milk: 55.23μg/ml local, 8.86μg/ml commercial; eggs: 46.88μg/g local, 11.44μg/g commercial). Molecular docking confirmed penicillin's strong ubiquitin binding (-5.7 kcal/mol), correlating with experimental data and validating computational approaches for residue prediction. Findings emphasize the need for stricter veterinary oversight and withdrawal period compliance.

Dimensions

Published

2025-04-27

How to Cite

Abdulsalam Saleh, Rihab Ramadhan Farhat, Zafir AM Zafir, Randa Mahmoud, Mohammd Abd Alati, & Hajir Othman. (2025). Evaluation of veterinary antibiotic residues in commercial and local farms in Al-Bayda city, Libya (2024-2025), with a molecular docking study to analyze their effect on target microbial proteins. African Journal of Advanced Pure and Applied Sciences (AJAPAS), 4(2), 219–226. Retrieved from https://aaasjournals.com/index.php/ajapas/article/view/1277

Issue

Volume 4, Issue 2, April- June 2025

Section

Articles
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