Spectrophotometric determination of hydrochlorothiazide using charge transfer interactions with alizarin red s reagent

Authors

  • Ikhlass Thanone Al-Allaf Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq
  • Nabeel Sabeeh Othman Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq
  • Amer Thanoon Al-Taee Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq

Keywords:

Hydrochlorothiazide, Alizarin Red S, Charge transfer, Tablets

Abstract

The present work included using transferred charge complex reaction in the assay of Hydrochlorothiazide using Alizarin Red S as an electron acceptor. The method is based on direct spectrophotometric estimation of hydrochlorothiazide by its reaction with Alizarin Red –S in absolute methanol media through forming a red charge transfer complex solution that gives a maximum absorption at 537 nm. The relation between concentration and absorbance was linear within the concentration range of 0.1-2.5 μg / ml with the molar absorption coefficient equal to 1.2774 × 105 l. mol-1. cm-1. The method was successfully applied to estimate hydrochlorothiazide in its pharmaceutical preparations (tablets).

 

 

Author Biographies

Ikhlass Thanone Al-Allaf, Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq

 

 

Nabeel Sabeeh Othman, Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq

 

 

Amer Thanoon Al-Taee, Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq

 

 

Dimensions

Published

2023-03-02

How to Cite

Ikhlass Thanone Al-Allaf, Nabeel Sabeeh Othman, & Amer Thanoon Al-Taee. (2023). Spectrophotometric determination of hydrochlorothiazide using charge transfer interactions with alizarin red s reagent. African Journal of Advanced Pure and Applied Sciences (AJAPAS), 2(1), 156–164. Retrieved from https://aaasjournals.com/index.php/ajapas/article/view/266